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Image Search Results
Journal: Current protocols in cytometry
Article Title: CyTOF Measurement of Immunocompetence across Major Immune Cell Types
doi: 10.1002/cpcy.27
Figure Lengend Snippet: Internet Resources
Article Snippet: Dry ice and regular ice Complete medium (see recipe) Pierce Universal Nuclease, 25kU (ThermoFisher Scientific, Catalog #88701) CyPBS (see recipe) CyFACS (see recipe) Brefeldin A (Sigma-Aldrich, Catalog #B7651) Monensin 1000× (BioLegend, Catalog #420701) Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, Catalog #P8139) Ionomycin (Sigma-Aldrich, Catalog #I0634) EDTA, 0.5M (Gibco, Catalog #15575) Maleimide-DOTA loaded with 115 In, 5mg/ml (Macrocyclics, Catalog #B-272) Paraformaldehyde, 16% w/v (Alfa Aesar, Catalog #43368) 10× permeabilization buffer (eBioscience, Catalog #00-8333-56) Cell ID Ir-Intercalator ( 193 Ir/ 195 Ir) 1000× (Fluidigm, Catalog #201192A)
Techniques: Antibody Labeling, Staining